Platform and Cell Biology Team

About the Company

Acquired by GSK, the team focuses on building an advanced oligonucleotide platform using the ROSALIND selection process, enabling breakthrough developments in RNA-based therapeutics. The platform facilitates high-throughput screening and data-driven approaches for optimizing gene-targeted drugs.

My role

• My Role
• Automation of High-Throughput Screenings
  • Spearheading the automation of high-throughput screenings using the Tecan Fluent780 system.
  • Writing complex scripts for NGS prep, knockdown studies, luminescence assays, and sample preparation.
  • Managing teaching labware, setting up liquid classes, creating subroutines, and streamlining workflows.
  • Completed Intermediate and Advanced FluentControl certification courses to enhance workflow efficiency.
• Platform Team – Toxicity Assay Development
  • Developing high-throughput toxicity assays to screen compounds causing alternative complement pathway activation responses.
  • Studying protein interactions and ASO affinity, performing NGS prep and sequencing, and conducting corresponding biochemical assays using ELISA.
  • Implementing flow cytometry-based approaches to study liver toxicity and using sorted cells for further sequencing applications.
• Cell Biology
  • Optimizing luminescence-based caspase assays for apoptosis studies.
  • Performing qPCR for knockdown validation and screening large-scale samples in 384-well formats for high-throughput screens.
• Workflow Automation and Data Analysis
  • Automating workflows from liquid handling to data analysis using Python scripts to ensure reproducibility and efficiency.
• Data Presentation and SOP Development
  • Presenting experimental data regularly to cross-functional teams to drive decision-making.
  • Writing Standard Operating Procedures (SOPs) to standardize workflows and improve lab operations.